The laboratories and blood banks currently use Cyanmethaemoglobin method for the determination of haemoglobin levels in the blood. This reagent contains low concentration of Potassium cyanide which is toxic and therefore the laboratories are advised not to pipette this reagent with mouth.

Advantage of Alkaline Haematin D Reagent Kit is that the chemicals used are practically non-toxic and non-hazardous and therefore do not require special attention for analysis and disposal.

Reagent and Calibrators have long life and remain stable over long periods without affecting the climatic conditions.

Serum lipids or proteins do not interfere in the estimation.

When a blood sample is added to an alkaline solution containing a non-ionic detergent, the haemoglobin is converted to alkaline haematin which is a stable colour compound. The absorbance of alkaline haematin is measured using Haemoglobinometer or a Photometer having concentration mode or semi-automated clinical chemistry analyzer.

Shelf life of reagent and calibrators is at least one year at room temperature.

Dispense 2.0 ml of reagent in a clean tube preferably using a dispenser for accurate dispensing and add accurately 20 μl of whole blood (capillary blood or anticoagulated venous blood). Mix well and read between 540 nm and 580 nm after calibrating with the standards supplied. The color produced is very stable.