Alkaline Hematin-D (AHD) method (Cyanide-free)

Hemoglobin Reagents Alkaline Hematin-D (AHD) method (Cyanide-free)
Alkaline Hematin-D (AHD) method (Cyanide-free)

Hemoglobin Reagents

Alkaline Hematin-D (AHD) method (Cyanide-free)

PRINCIPLE:

When a blood sample is added to an alkaline solution containing a non-ionic detergent, haemoglobin is converted to alkaline haematin which is a stable colour compound. The absorbance of alkaline haematin is measured using Colorimeter, Haemoglobin Meter or Clinical Chemistry Analyser..

STORAGE AND STABILITY

Shelf life of one year at room temperature, for reagent and standards.

PROCEDURE

Dispense 2.0 mL of the reagent in a clean test tube and add accurately 20 μL of whole blood. Mix well and read at 578nm (540 nm – 580 nm). The colour produced is very stable.

Category: Hemoglobin Reagents

Description

Hemoglobin Reagents Alkaline Hematin-D (AHD) method (Cyanide-free)
Hemoglobin Reagents
Alkaline Hematin-D (AHD) method (Cyanide-free)
DRABKIN REAGENTDIRECT HAEMOGLOBIN REAGENT
Hazardous and ToxicNon Hazardous and Non Toxic
Requires 5 mL for each test using 20 μL sampleRequires 2 mL for each test using 20 μL sample
Final colour formed is not very stable and takes at least 5 minutes to develop colourTakes only 1 minute to develop colour and the colour complex formed is very stable
Only 1 artificial standard provided for calibrationLinearity has been checked for 0.5g/dL to 30.0g/dL. Two stable standards with long life

* Specifications are subject to change without prior notice.

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