When a blood sample is added to an alkaline solution containing a non-ionic detergent, haemoglobin is converted to alkaline haematin which is a stable colour compound. The absorbance of alkaline haematin is measured using Colorimeter, Haemoglobin Meter or Clinical Chemistry Analyser..
STORAGE AND STABILITY
Shelf life of one year at room temperature, for reagent and standards.
Dispense 2.0 mL of the reagent in a clean test tube and add accurately 20 μL of whole blood. Mix well and read at 578nm (540 nm – 580 nm). The colour produced is very stable. Category: Hemoglobin Reagents
DIRECT HAEMOGLOBIN REAGENT
Hazardous and Toxic
Non Hazardous and Non Toxic
Requires 5 mL for each test using 20 μL sample
Requires 2 mL for each test using 20 μL sample
Final colour formed is not very stable and takes at least 5 minutes to develop colour
Takes only 1 minute to develop colour and the colour complex formed is very stable
Only 1 artificial standard provided for calibration
Linearity has been checked for 0.5g/dL to 30.0g/dL. Two stable standards with long life
* Specifications are subject to change without prior notice.